Western Blot Ag Signal Enhancer
Size: 500mL
Model :
BR560-500
Western Blot Ag Signal Enhancer modifies the surface structure of transfer membranes (NC or PVDF), increases the exposure of target proteins, and thus produces more antibody-antigen bindings.- Sensitivity is enhanced by 3 to 10 times.
- Usage of primary antibodies can be reduced.
- Detection of low concentration protein can be improved.
INSTRUCTIONS:
★ 30mL Western Blot Ag Signal Enhancer for 1 sheet of 8 x 10 cm membrane ★- After transfer, incubate NC or PVDF transfer membranes with Western Blot Ag Signal Enhancer at room temperature for 20 minutes.
- Remove the transfer membrane from Western Blot Ag Signal Enhancer, and wash by TBST buffer twice, 5 minutes each time.
- Continue following blocking, incubation, etc. steps of western blot.
Chemicals
- Instruments
- Chemicals
- Western Blot Specific
- Prestained Protein Marker
- Stripping Buffer
- Transfer Buffer (10X)
- Resolving Gel Buffer (4X)
- Stacking Gel Buffer (4X)
- PBS Buffer, pH7.4 (10X)
- SDS Buffer (10%)
- TBS Buffer (10X)
- TBST Buffer (10X)
- TG Buffer (10X)
- TGS Buffer (10X)
- Western Blot Ag Signal Enhancer
- Western Blot Ab Signal Enhancer
- Western Blot Protein-Free Blocker
- Protein Sample Buffer (5X)
- Ponceau S Staining Solution
- DNA Electrophoresis Related
- Immunohistochemistry Specific
- Gerneral Use
- RNA Extraction Kits
- Western Blot Specific
- Consumables