Pre-stained Protein Marker (9-180 kDa) is a ready to use three-color protein standard with 10 pre-stained proteins covering a wide range of molecular weights from 10 to 180 kDa (9 to 170 kDa in Bis-Tris (MOPS) buffer and 10 to 170 kDa in Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-Glycine buffer).
Pre-stained Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electro-phoresis, verification of Western transfer efficiency on membranes, (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
The marker is supplied in gel loading buffer and is ready to use. Do NOT heat, dilute, or add reducing agents before loading.